Validated RP – HPLC Method for the Estimation of Denosumab in Formulation
Abstract A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Denosumab in tablet dosage form. Isocratic elution at a flow rate of 1.0ml/min was employed on a symmetry C18 (250×4.6mm, 5μm in particle size) at ambient temperature. The mobile phase consisted of methanol: water: O.P.A 90:10:01 (V/V/V)….