In Vitro Plant Regeneration from Shoot Tip and Nodal Explants of Rhinacanthus Nasutus (L.) Kurz

A simple, precise and accurate RP-HPLC method was developed and validated for rapid assay of Gemcitabinein tablet dosage form. Isocratic elution at a flow rate of 1ml/min was employed on a symmetry Chromosil C18 (250x4.6mm, 5µm in particle size) at ambient temperature. The mobile phase consisted of Acetonitril: Methanol 82: 18 v/v, (PH 5.9 with 0.1M Phosphate buffer). The UV detection wavelength was 252nm and 20µl sample was injected. The retention time for Gemcitabinewas 6.89min. The percentage RSD for precision and accuracy of the method was found to be less than 2%. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of Gemcitabinein tablet dosage form and bulk drug.