New method development and validation for Simultaneous determination of 3 terpenoids (Terpineol, Limonene, α-Terpinene) in Black cardamom by HPLC

A HPLC method was developed and validated for simultaneous determination of Terpineol, Limonene, α-Terpinenein Black cardamom using a Hypersil bds C18column (250 mm×4.6 mm, 5 μm) with the mobile phase consisting of Methanol and 0.1% triethylamine,0.05M KH2PO4used as mobile phase in the ration of 45%,10%,45%  The linear ranges of three compounds were determined and method validation was evaluated completely. The established method is rapid and accurate with high repeatability.