A New L.C method development and validation for analysis of Atvimopin in Formulations.

A simple, rapid and precise reverse phase high performance liquid chromatography method was developed for the analysis of Atvimopin Chromatographic separation of  Atvimopin was performed by using a waters C18 column (250 x 4.6mm, 5 µm) as stationary phase with a mobile phase comprising of Methanol:Acetonitrile80:20 (v/v/v) at a flow rate of 1.0ml/min and UV detection at 261 nm and 20µl sample was injected. The linearity of Atvimopin is in the range of 40 ppm to 100 ppm. The proposed method was found to be accurate, precise and rapid for the analysis of Atvimopin. The retention time for Atvimopinwas 5.3 min. The percentage RSD for precision and accuracy of the method was found to be less than 2%. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of Atvimopintablet dosage form and bulk drug.