HPLC and UV Spectrophotometric Estimation of Pyrazinamide in Pharmaceutical Formulations

Pyrazinamide is an antituberculosis agent. It works by killing or preventing the growth of certain bacteria that cause tuberculosis. HPLC and UV method was employed for the estimation of Pyrazinamide in pharmaceutical formulations. A mixture of Methanol: Water in the ratio of 80:20 (v/v/v) at a pH of 4.8 in isocratic conditions and separation was achieved on Zodiac C18 column (250 X 4.6 mm, 5μ) at ambient temperature. UV detection was monitored at a wavelength of 240nm. In these conditions, the drug elutes at a retention time of 5.2 min for sample and standard solutions. In UV method, the standard drug was diluted using methanol solvent and maximum absorbance was found to be 271nm and the analysis was carried in this wavelength. Beers law limit was found to be in the range of 25-150µg/ml and 1-6µg/ml for HPLC and UV methods respectively. Macrozide (150mg) brand was used for formulation analysis and the % assay was found to be more than 98% for both the HPLC and UV methods in all the brands in the study.