Estimation of Caffeine in Chocolates and Soft drinks by High Pressure Liquid Chromatographic technique

Caffeine is a central nervous system stimulant of the methylxanthine class of psychoactive drugs. It is the world's most widely consumed psychoactive drug. High Pressure Liquid Chromatographic technique was employed to study caffeine contents in different coal drinks and chocolates. The concentration of caffeine in 1gr. Coffee bite was found to be 1887.5µg, 70.193µg in Galaxy smooth, 60.590µg in Dairy milk. We also estimate the amount of caffeine in every 50ml of soft drinks also. Results conforms that Pepsi contain 228.973µg, 85.878µg in coco cola. All the samples in the study contain high amount of caffeine. The intake of caffeine may results nurvesness, restlessness, and nervousness, increased heartbeat, nausea, anxiety, heart palpitations, insomnia, sweating, dizziness, vomiting and cardiac arrest.