A rapid, sensitive and selective analytical Liquid chromatography tandem mass spectrometry method was developed and validated for the determination Telmisartan and Hydrochlorothiazide in human plasma. Liquid-liquid extraction was used for sample preparation and analysis, followed by liquid chromatography tandem spectrometric analysis and an electrospray-ionization interface. Compounds were analyzed on a Aquasil-C18 (250×4.6mm×5µm) column with the mobile phase of pH 4.5 Acetate buffer solution, Methanol and Acetonitrile in the ratio of 60:20:20 (v/v) in isocratic condition at a ﬂow rate of 0.5m L/min for 10min. a retention time of 4.39min and 5.73min were observed for Telmisartan and Hydrochlorothiazide respectively. The method was validated as per ICH guidelines as Linearity, precision, accuracy, recovery and different stability studies. All the results obtained were found to be within the acceptance limit. Hence the developed LC/MS/MS method was successfully applied for the determination of Telmisartan and Hydrochlorothiazide in human plasma.
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