Development and Validation of RP-HPLC Method for the Simultaneous Estimation of Avibactam and Ceftazidime in Bulk drug and injection dosage Form

The aim of the present work was to develop simple, shorter and effective HPLC method with UV detection (255 nm) and subsequent validation for the simultaneous determination of Avibactam and Ceftazidime in marketed tablet samples. The mobile phase consisted of 1% OPA: Acetonitrile 55:45 (v/v) [pH 5.5], an isocratic elution at a flow rate of 1.5 ml/min at ambient temperature. The detection was carried out at 255nm. Retention times were found to be 3.00 min for Avibactam and 5.05 min for Ceftazidime. Good linear relation was observed with in a concentration range of 5-30µg/ml for Avibactam and 10-60µg/ml for Ceftazidime with high r2 value. The developed method was validated as per ICH guide lines. The developed methods were found to be precise and accurate for the estimation of Avibactam and Ceftazidime in pharmaceutical dosage forms.